sumo 2 3 (Proteintech)
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sumo 2 3
Sumo 2 3, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sumo2+3/SUMO2%2F3+Antibody/10__34133_slash_research__1243-286-17-19
Average 94 stars, based on 27 article reviews
Sumo 2 3, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sumo2+3/SUMO2%2F3+Antibody/10__34133_slash_research__1243-286-17-19
Average 94 stars, based on 27 article reviews
sumo 2 3 - by Bioz Stars,
2026-10
94/100 stars
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Western Blot:Article Title: IFI204 in microglia mediates traumatic brain injury-induced mitochondrial dysfunction and pyroptosis via SENP7 interaction. Article Snippet: Data processing was performed using FlowJo 10.8.1 (BD Biosciences, CA), and results are presented in terms of red fluorescence/green fluorescence ratio. .. Western blot Briefly, protein was applied to electrophoresis, electroporation, blocking, and incubation with the primary antibodies: IFI204 (Abcam), GSDMD (Abcam), GSDMD-N (Cell Signaling Technology, CST), Pro caspase-1 (Adipogen), caspase-1 p20 (Cell Signaling Technology), SENP7 (Santa Cruz Biotechnologies), Article Title: Mild microwave hyperthermia promotes mitotic catastrophe, induces time-delayed cGAS-STING activation and restores sensitivity to anti-PDL1 therapy in Pan02 pancreatic cancer model Article Snippet: The transfection reagents Lipofectamine 2000TM and JetPRIMETM were purchased from Invitrogen (Carlsbad, CA, USA) and Polyplus (Illkirch, France). .. The following antibodies were used for western blotting and immunostaining: anti-human cGAS (Cell Signaling), anti-mouse cGAS (SantaCruz), STING (Novus Biological), phospho-IRF3 Ser-386 (Cell Signaling), phospho-IRF3 Ser-396 (Cell Signaling), IRF-3 (SantaCruz), phospho-TBK1 Ser172 (Cell Signaling), TBK1 (SantaCruz), phospho-H2A.X Ser139 (Cell Signaling), PD-L1 (Cell Signaling), CD11c (Cell Signaling), CD8α (Cell Signaling), F4/80 (Proteintech), CD163 (Proteintech), CD86 (Novus Biologicals), FoxP3 (Cell Signaling), Gr1 (Invitrogen), GranzymeB (Invitrogen), Tim3 (Invitrogen), phospho-STAT1 Tyr701 (Cell Signaling), and IFNβ, CCL5, CXCL10, CD56, MICA, HMGB1, SUMO1, Article Title: Mild microwave hyperthermia promotes mitotic catastrophe, induces time-delayed cGAS-STING activation and restores sensitivity to anti-PDL1 therapy in Pan02 pancreatic cancer model Article Snippet: The transfection reagents Lipofectamine 2000 TM and JetPRIME TM were purchased from Invitrogen (Carlsbad, CA, USA) and Polyplus (Illkirch, France). .. The following antibodies were used for western blotting and immunostaining: anti-human cGAS (Cell Signaling), anti-mouse cGAS (SantaCruz), STING (Novus Biological), phospho-IRF3 Ser-386 (Cell Signaling), phospho-IRF3 Ser-396 (Cell Signaling), IRF-3 (SantaCruz), phospho-TBK1 Ser172 (Cell Signaling), TBK1 (SantaCruz), phospho-H2A.X Ser139 (Cell Signaling), PD-L1 (Cell Signaling), CD11c (Cell Signaling), CD8α (Cell Signaling), F4/80 (Proteintech), CD163 (Proteintech), CD86 (Novus Biologicals), FoxP3 (Cell Signaling), Gr1 (Invitrogen), GranzymeB (Invitrogen), Tim3 (Invitrogen), phospho-STAT1 Tyr701 (Cell Signaling), and IFNβ, CCL5, CXCL10, CD56, MICA, HMGB1, SUMO1, Electrophoresis:Article Title: IFI204 in microglia mediates traumatic brain injury-induced mitochondrial dysfunction and pyroptosis via SENP7 interaction. Article Snippet: Data processing was performed using FlowJo 10.8.1 (BD Biosciences, CA), and results are presented in terms of red fluorescence/green fluorescence ratio. .. Western blot Briefly, protein was applied to electrophoresis, electroporation, blocking, and incubation with the primary antibodies: IFI204 (Abcam), GSDMD (Abcam), GSDMD-N (Cell Signaling Technology, CST), Pro caspase-1 (Adipogen), caspase-1 p20 (Cell Signaling Technology), SENP7 (Santa Cruz Biotechnologies), Article Title: IFI204 in microglia mediates traumatic brain injury-induced mitochondrial dysfunction and pyroptosis via SENP7 interaction Article Snippet: Data processing was performed using FlowJo 10.8.1 (BD Biosciences, CA), and results are presented in terms of red fluorescence/green fluorescence ratio. .. Briefly, protein was applied to electrophoresis, electroporation, blocking, and incubation with the primary antibodies: IFI204 (Abcam), GSDMD (Abcam), GSDMD-N (Cell Signaling Technology, CST), Pro caspase-1 (Adipogen), caspase-1 p20 (Cell Signaling Technology), SENP7 (Santa Cruz Biotechnologies), Electroporation:Article Title: IFI204 in microglia mediates traumatic brain injury-induced mitochondrial dysfunction and pyroptosis via SENP7 interaction. Article Snippet: Data processing was performed using FlowJo 10.8.1 (BD Biosciences, CA), and results are presented in terms of red fluorescence/green fluorescence ratio. .. Western blot Briefly, protein was applied to electrophoresis, electroporation, blocking, and incubation with the primary antibodies: IFI204 (Abcam), GSDMD (Abcam), GSDMD-N (Cell Signaling Technology, CST), Pro caspase-1 (Adipogen), caspase-1 p20 (Cell Signaling Technology), SENP7 (Santa Cruz Biotechnologies), Article Title: IFI204 in microglia mediates traumatic brain injury-induced mitochondrial dysfunction and pyroptosis via SENP7 interaction Article Snippet: Data processing was performed using FlowJo 10.8.1 (BD Biosciences, CA), and results are presented in terms of red fluorescence/green fluorescence ratio. .. Briefly, protein was applied to electrophoresis, electroporation, blocking, and incubation with the primary antibodies: IFI204 (Abcam), GSDMD (Abcam), GSDMD-N (Cell Signaling Technology, CST), Pro caspase-1 (Adipogen), caspase-1 p20 (Cell Signaling Technology), SENP7 (Santa Cruz Biotechnologies), Blocking Assay:Article Title: IFI204 in microglia mediates traumatic brain injury-induced mitochondrial dysfunction and pyroptosis via SENP7 interaction. Article Snippet: Data processing was performed using FlowJo 10.8.1 (BD Biosciences, CA), and results are presented in terms of red fluorescence/green fluorescence ratio. .. Western blot Briefly, protein was applied to electrophoresis, electroporation, blocking, and incubation with the primary antibodies: IFI204 (Abcam), GSDMD (Abcam), GSDMD-N (Cell Signaling Technology, CST), Pro caspase-1 (Adipogen), caspase-1 p20 (Cell Signaling Technology), SENP7 (Santa Cruz Biotechnologies), Article Title: SENP3/FIS1-regulated PFC neural mitochondrial fragmentation underlies the mechanism of electroacupuncture attenuating depressive behavior in CUMS mice Article Snippet: .. After blocking with BSA/TBST at room temperature for 3 h, the membranes were incubated with primary antibodies, including DRP1 (1:1500, 12957-1-Ap, Proteintech, Chicago, USA), MFF (1:4000, 66527-1-Ig, Proteintech), FIS1 (1:1000, SAB2702049, Sigma-Aldrich), Article Title: IFI204 in microglia mediates traumatic brain injury-induced mitochondrial dysfunction and pyroptosis via SENP7 interaction Article Snippet: Data processing was performed using FlowJo 10.8.1 (BD Biosciences, CA), and results are presented in terms of red fluorescence/green fluorescence ratio. .. Briefly, protein was applied to electrophoresis, electroporation, blocking, and incubation with the primary antibodies: IFI204 (Abcam), GSDMD (Abcam), GSDMD-N (Cell Signaling Technology, CST), Pro caspase-1 (Adipogen), caspase-1 p20 (Cell Signaling Technology), SENP7 (Santa Cruz Biotechnologies), Incubation:Article Title: IFI204 in microglia mediates traumatic brain injury-induced mitochondrial dysfunction and pyroptosis via SENP7 interaction. Article Snippet: Data processing was performed using FlowJo 10.8.1 (BD Biosciences, CA), and results are presented in terms of red fluorescence/green fluorescence ratio. .. Western blot Briefly, protein was applied to electrophoresis, electroporation, blocking, and incubation with the primary antibodies: IFI204 (Abcam), GSDMD (Abcam), GSDMD-N (Cell Signaling Technology, CST), Pro caspase-1 (Adipogen), caspase-1 p20 (Cell Signaling Technology), SENP7 (Santa Cruz Biotechnologies), Article Title: SENP3/FIS1-regulated PFC neural mitochondrial fragmentation underlies the mechanism of electroacupuncture attenuating depressive behavior in CUMS mice Article Snippet: .. After blocking with BSA/TBST at room temperature for 3 h, the membranes were incubated with primary antibodies, including DRP1 (1:1500, 12957-1-Ap, Proteintech, Chicago, USA), MFF (1:4000, 66527-1-Ig, Proteintech), FIS1 (1:1000, SAB2702049, Sigma-Aldrich), Article Title: Multi-level intervention of Tangluoning on mitochondrial thioredoxin deficiency in Schwann cells ameliorates diabetic peripheral neuropathy. Article Snippet: Background: Diabetic peripheral neuropathy (DPN), the most prevalent complication of diabetes, currently lacks disease-modifying therapies.. While Tangluoning recipe (TLN)-a traditional Chinese herbal medicine derived from Huangqi Guizhi Wuwu decoction—demonstrates therapeutic efficacy against DPN symptoms, its mechanism remained elusive.. This study pioneers two transformative advances: Uncovering a previously unrecognized pathogenic axis linking mitochondrial thioredoxin (Trx2) deficiency to DPN demyelination; Establishing a novel dual-target therapeutic strategy that concurrently rescues mitochondrial dysfunction and lipid metabolic Article Title: IFI204 in microglia mediates traumatic brain injury-induced mitochondrial dysfunction and pyroptosis via SENP7 interaction Article Snippet: Data processing was performed using FlowJo 10.8.1 (BD Biosciences, CA), and results are presented in terms of red fluorescence/green fluorescence ratio. .. Briefly, protein was applied to electrophoresis, electroporation, blocking, and incubation with the primary antibodies: IFI204 (Abcam), GSDMD (Abcam), GSDMD-N (Cell Signaling Technology, CST), Pro caspase-1 (Adipogen), caspase-1 p20 (Cell Signaling Technology), SENP7 (Santa Cruz Biotechnologies), Immunostaining:Article Title: Mild microwave hyperthermia promotes mitotic catastrophe, induces time-delayed cGAS-STING activation and restores sensitivity to anti-PDL1 therapy in Pan02 pancreatic cancer model Article Snippet: The transfection reagents Lipofectamine 2000TM and JetPRIMETM were purchased from Invitrogen (Carlsbad, CA, USA) and Polyplus (Illkirch, France). .. The following antibodies were used for western blotting and immunostaining: anti-human cGAS (Cell Signaling), anti-mouse cGAS (SantaCruz), STING (Novus Biological), phospho-IRF3 Ser-386 (Cell Signaling), phospho-IRF3 Ser-396 (Cell Signaling), IRF-3 (SantaCruz), phospho-TBK1 Ser172 (Cell Signaling), TBK1 (SantaCruz), phospho-H2A.X Ser139 (Cell Signaling), PD-L1 (Cell Signaling), CD11c (Cell Signaling), CD8α (Cell Signaling), F4/80 (Proteintech), CD163 (Proteintech), CD86 (Novus Biologicals), FoxP3 (Cell Signaling), Gr1 (Invitrogen), GranzymeB (Invitrogen), Tim3 (Invitrogen), phospho-STAT1 Tyr701 (Cell Signaling), and IFNβ, CCL5, CXCL10, CD56, MICA, HMGB1, SUMO1, Article Title: Mild microwave hyperthermia promotes mitotic catastrophe, induces time-delayed cGAS-STING activation and restores sensitivity to anti-PDL1 therapy in Pan02 pancreatic cancer model Article Snippet: The transfection reagents Lipofectamine 2000 TM and JetPRIME TM were purchased from Invitrogen (Carlsbad, CA, USA) and Polyplus (Illkirch, France). .. The following antibodies were used for western blotting and immunostaining: anti-human cGAS (Cell Signaling), anti-mouse cGAS (SantaCruz), STING (Novus Biological), phospho-IRF3 Ser-386 (Cell Signaling), phospho-IRF3 Ser-396 (Cell Signaling), IRF-3 (SantaCruz), phospho-TBK1 Ser172 (Cell Signaling), TBK1 (SantaCruz), phospho-H2A.X Ser139 (Cell Signaling), PD-L1 (Cell Signaling), CD11c (Cell Signaling), CD8α (Cell Signaling), F4/80 (Proteintech), CD163 (Proteintech), CD86 (Novus Biologicals), FoxP3 (Cell Signaling), Gr1 (Invitrogen), GranzymeB (Invitrogen), Tim3 (Invitrogen), phospho-STAT1 Tyr701 (Cell Signaling), and IFNβ, CCL5, CXCL10, CD56, MICA, HMGB1, SUMO1, |
![(A) HFF cells stably transduced with control sgRNA or sgRNA targeting UBE2I were infected with HSV-1 ICP0 RFm (MOI = 5). WCLs were analyzed by immunoblotting at 24 h post-infection, followed by densitometric quantification of band intensities. (B) HFF cells were infected with HSV-1 ICP0 RFm (MOI = 5) for 24 h. Denaturing immunoprecipitation was performed using anti-SUMO1 or <t>anti-SUMO2/3</t> antibodies, and both input and immunoprecipitated samples were analyzed by immunoblotting. (C) U2OS cells stably transduced with control sgRNA or sgRNA targeting SUMO1 were infected with HSV-1 ΔICP0 (MOI = 5) for 24 h. WCLs were analyzed by immunoblotting, followed by densitometric quantification of band intensities. (D) U2OS cells stably transduced with control sgRNA or sgRNA targeting SUMO2 and SUMO3 were infected with HSV-1 ΔICP0 (MOI = 5) for 24 h. WCLs were analyzed by immunoblotting, followed by densitometric quantification of band intensities. (E) HEK293T cells were co-transfected with the indicated plasmids for 24 h and subsequently infected with HSV-1 ICP0 RFm (MOI = 5) for 24 h. Denaturing immunoprecipitation was performed, and both input and immunoprecipitated samples were analyzed by immunoblotting. (F) SMCHD1 -knockdown U2OS cells reconstituted with SMCHD1 WT, or the indicated mutants (Δhinge, K1848R, K1852R, K1872R, or the 3KR mutant [K1848R/K1852R/K1872R]) were infected with HSV-1 ΔICP0 (MOI = 5) for 24 h. WCLs were analyzed by immunoblotting, followed by densitometric quantification of band intensities. Data are presented as mean ± SD from three independent experiments (n = 3). Statistical significance was determined by one-way ANOVA or unpaired two tailed Student’s t test. p value: *, p < 0.05; **, p < 0.01; ***, p < 0.005. N.S.: no significance.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_3348/pmc13313348/pmc13313348__ppat.1014371.g004.jpg)
